[{
  "type": "article-journal",
  "title": "EFFECTS OF 30 DAY SUB-LETHAL EXPOSURE OF CADMIUM AND LEAD MIXTURE ON DNA DAMAGE IN FISH",
  "author": [
    {
      "family": "Ambreen",
      "given": ""
    },
    {
      "family": "Javed",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2016]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "26",
  "issue": "3",
  "page": "674-679",
  "DOI": "NA",
  "abstract": "<p>The present study was undertaken to examine the DNA damage induced by binary metal mixture (cadmium-lead) in peripheral blood erythrocytes of freshwater carp,&nbsp;<em>Cyprinus carpio</em>&nbsp;by using Comet bioassay. The 96-hr LC50 value of mixture was estimated for 180-old fingerlings of&nbsp;<em>Cyprinus carpio&nbsp;</em>in a static system and then four sub-lethal concentrations viz. SL-I (10.42mgL-1), SL-II (7.81mgL-1), SL-III (6.25mgL-1) and SL-IV (5.21mgL-1) were calculated and fishwere exposed to these concentrations, separately in glass aquaria for 30 days along with negative and positive control at constant water temperature (30&ordm;C), pH (7.75) and total hardness (225mgL-1). Peripheral erythrocytes were sampled after 30 day exposure for DNA damage assessment. Statistically significant effects were observed at sub-lethal concentrations in-terms of percentage of DNA damage, cumulative tail lengths (&micro;m) and genetic damage index. Concentration dependent response was observed in fish erythrocytes with induction of maximum DNA damage, due to positive control, followed by at highest concentration (SL-I) of mixture. This study also concluded that comet bioassay can be used for in-vivo experiments, using fish as a model for the screening of genotoxic and mutagenic pollutants in aquatic environment.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-92"
}]