[{
  "type": "article-journal",
  "title": "LENS PROTEOMICS: EXPLORING ADAPTIVE CONFLICT IN UROMASTYX HARDWICKII LENS PROTEINS",
  "author": [
    {
      "family": "Atta",
      "given": ""
    },
    {
      "family": "Hashim",
      "given": ""
    },
    {
      "family": "Zarina",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2016]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "26",
  "issue": "1",
  "page": "261-268",
  "DOI": "NA",
  "abstract": "<p>During last decade, proteomics has emerged as a powerful tool for high throughput screening, identification and quantification of proteins from biological samples. In current study, we have employed proteomics technique to explore proteins from eye lenses of&nbsp;<em>Uromastyx hardwickii,&nbsp;</em>an Indian spiny-tailed lizard. Water soluble and insoluble proteins from&nbsp;<em>Uromastyx hardwickii</em>&nbsp;lens were separated and characterized by 2-DE (two dimensional electrophoresis). After in-gel digestion, well resolved spots were identified by nano- liquid chromatography-electrospray tandem mass spectrometry (nLC-MS/MS). We have identified &alpha;A-, &beta;A2-, &beta;A4-, &beta;B2- and &beta;B3-crystallins as ubiquitous crystallins and &delta;-, &tau;-crystallins as taxon-specific lens crystallins in water soluble fraction. &alpha;A- and &beta;B3-crystallins were found to be most abundant water soluble proteins in&nbsp;<em>Uromastyx hardwickii&nbsp;</em>lens. Among non-crystallins (cellular proteins), &beta;-actin was identified. From water insoluble fraction, vimentin, gelsolin, gamma enolase like protein, filensin like protein and ATP synthase subunit B were identified. Our results illustrate advantage of proteomics technology for identification of lens proteins. Furthermore, the study provides distribution patterns of ubiquitous and taxon specific crystallins from&nbsp;<em>Uromastyx hardwickii</em> that is likely to be useful in understanding evolutionary lineage of this organism.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-33"
}]