[{
  "type": "article-journal",
  "title": "MOLECULAR CLONING AND EXPRESSION ANALYSIS OF A PUTATIVE E CLASS MADS-BOX GENE, GbSEP, FROM GINKGO BILOBA",
  "author": [
    {
      "family": "Cheng",
      "given": ""
    },
    {
      "family": "Cheng",
      "given": ""
    },
    {
      "family": "Xu",
      "given": ""
    },
    {
      "family": "Ye",
      "given": ""
    },
    {
      "family": "Wang",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2016]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "26",
  "issue": "1",
  "page": "253-260",
  "DOI": "NA",
  "abstract": "<p>MADS-box gene family plays an important role in the development of floral organs in plants. A full-length cDNA of MADS-box gene, named as&nbsp;<em>GbSEP</em>(Genbank accession numberKJ630504), was cloned by RT-PCR from female flower of&nbsp;<em>Ginkgo biloba</em>. The cDNA sequence of&nbsp;<em>GbSEP</em>&nbsp;contained an ORF of 756 bp encoding 252 amino acid residues. The comparison between cDNA and genomic sequences of&nbsp;<em>GbSEP</em>&nbsp;indicated no intron in the genomic DNA sequence of this gene. Protein multi-alignment analysis indicated that GbSEP shared high homology with other SEP family proteins from other plants including&nbsp;<em>Pinus radiate</em>&nbsp;(PrMADS3),&nbsp;<em>Pinus resinosa&nbsp;</em>(PrMADS1),&nbsp;<em>Arabidopsis thaliana</em>&nbsp;(AtSEP4),&nbsp;<em>Alpinia hainanensis</em>&nbsp;(AhSEP3),&nbsp;<em>Lotus japonicas</em>&nbsp;(LjSEP),&nbsp;<em>Populus tomentosa</em>&nbsp;(PtSEP), and&nbsp;<em>Mangifera indica</em>&nbsp;(MiSEP3). Moreover, GbSEP contained a MADS-box and a K-box domain, typical conservative domains of plant MADS-box proteins. Phylogenetic tree clearly showed that GbSEP is classed into SEP subgroup of E class MADS-box protein family. Real-time PCR analysis revealed that expression levels of&nbsp;<em>GbSEP</em>&nbsp;gene in female and male flower were significantly higher than those in root, stem, and leaves, and that&nbsp;<em>GbSEP</em>&nbsp;expression level increased along with time of flower development, suggesting that&nbsp;<em>GbSEP</em>&nbsp;might be involved in development of reproductive organs in&nbsp;<em>G</em>.&nbsp;<em>biloba</em>.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-32"
}]