[{
  "type": "article-journal",
  "title": "PHYLOGENETIC GROUPING OF THE PATHOGENIC E. COLI ISOLATED FROM COMMERCIAL BROILER CHICKEN IN PAKISTAN",
  "author": [
    {
      "family": "Akhtar",
      "given": ""
    },
    {
      "family": "Rabbani",
      "given": ""
    },
    {
      "family": "Muhammad",
      "given": ""
    },
    {
      "family": "Younus",
      "given": ""
    },
    {
      "family": "Sheikh",
      "given": ""
    },
    {
      "family": "Akhtar",
      "given": ""
    },
    {
      "family": "Muhammad",
      "given": ""
    },
    {
      "family": "Rasool",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2016]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "26",
  "issue": "5",
  "page": "1242-1246",
  "DOI": "NA",
  "abstract": "<p><em>E. coli</em>&nbsp;isolates can be classified into four different phylogenetic groups on the basis of&nbsp;<em>chuA</em>,&nbsp;<em>yjaA</em>&nbsp;and TspE4.C2 biomarkers via multiplex PCR. Current study investigated the presence of various phylogenetic groups in clinical samples of collibacillosis from commercial broiler chicken in Pakistan. A total of 84&nbsp;<em>E. coli</em>&nbsp;isolates out of 200 swab sample (n = 25 cloacal; n = 27 fibrinous layer of peri-hepatitis of clinically infected broiler chicken; and n = 32 cloacal swabs from clinically healthy chicken) were recovered. Out of 25 pathogenic&nbsp;<em>E. coli</em>&nbsp;isolates from cloacal swabs, 12%, 4%, 72% and 0% isolates belonged to group A, B1, D and B2, respectively. Out of 27 pathogenic&nbsp;<em>E. coli</em>&nbsp;isolates from swab of fibrinous layer,18.5%, 29.6%, 44.4% and 0% isolates belonged to group A, B2, D and B1, respectively. While out of 32 non pathogenic&nbsp;<em>E. coli</em>&nbsp;isolates from healthy birds, 21.8%, 9.3%, 46.8% and 18.7% isolates belonged to group A, B2, D, and B1, respectively. There were 06 uncategorized&nbsp;<em>E. coli</em>&nbsp;isolates, which amplified both&nbsp;<em>yjaA&nbsp;</em>and TspE4.C2 biomarkers. This study helped to categorize the&nbsp;<em>E. coli</em> isolates into intestinal and extra intestinal origin on the basis of phylogenetic grouping.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-162"
}]