[{
  "type": "article-journal",
  "title": "A PLANT TRANSFORMATION VECTOR CONTAINING THE GENE dehD FOR THE DEVELOPMENT OF CULTIVARS RESISTANT TO MONOCHLOROACETIC ACID",
  "author": [
    {
      "family": "Mohamed",
      "given": ""
    },
    {
      "family": "Rahiman",
      "given": ""
    },
    {
      "family": "Wahab",
      "given": ""
    },
    {
      "family": "Zain",
      "given": ""
    },
    {
      "family": "Javed",
      "given": ""
    },
    {
      "family": "Huyop",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2016]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "26",
  "issue": "4",
  "page": "1133-1139",
  "DOI": "NA",
  "abstract": "<p><a id=\"_GoBack\" name=\"_GoBack\"></a>A<em>&nbsp;dehalogenase D</em>&nbsp;gene (<em>dehD</em>) capable of degrading monochloroacetic acid (MCA) has been previously isolated from&nbsp;<em>Rhizobium</em>&nbsp;sp. RC1 and characterized. The 804-bp<em>&nbsp;dehD</em>&nbsp;gene was inserted into pCAMBIA under the control of the cauliflower mosaic virus 35S promoter and designated pCAMdehD, with a total size of 10,592 bp. The pCAMdehD was introduced into tobacco (<em>Nicotiana benthamiana</em>) via&nbsp;<em>Agrobacterium</em>-mediated transformation. The integration and expression of&nbsp;<em>dehD</em>&nbsp;in&nbsp;<em>N. benthamiana&nbsp;</em>was confirmed by PCR and reverse transcription PCR, respectively. MCA-resistant transformants were selected in tissue cultures containing 60 &micro;g/L MCA. Analysis of plants using a leaf-painting assay revealed that transgenic&nbsp;<em>N. benthamiana&nbsp;</em>was resistant to4.0 g/L MCAcompared with 2.0 g/L for non-transformed controls. The use of&nbsp;<em>dehD</em>&nbsp;could thus be advantageous for herbicide-resistant plant breeding systems, and it is also a suitable marker gene for plant-transformation studies. To the best of our knowledge, this is the first report detailing transgenic&nbsp;<em>N. benthamiana</em>&nbsp;engineered with&nbsp;<em>dehD</em>&nbsp;from&nbsp;<em>Rhizobium&nbsp;</em>sp. RC1.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-148"
}]