[{
  "type": "article-journal",
  "title": "EFFECT OF L-CYSTEINE ON POST THAW QUALITY OF NILI RAVI BUFFALO (BUBALUS BUBALIS) BULL SPERMATOZOA CRYOPRESERVED IN TRIS CITRIC ACID EXTENDER",
  "author": [
    {
      "family": "Wadood",
      "given": ""
    },
    {
      "family": "Aleem",
      "given": ""
    },
    {
      "family": "Ijaz",
      "given": ""
    },
    {
      "family": "Ahmad",
      "given": ""
    },
    {
      "family": "Yousaf",
      "given": ""
    },
    {
      "family": "Mughal",
      "given": ""
    },
    {
      "family": "Mahmood",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2015]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "25",
  "issue": "1",
  "page": "301-303",
  "DOI": "NA",
  "abstract": "<p>This study was designed to determine effect of various concentrations of L- cysteine on frozen-thaw semen quality of Nili Ravi buffalo. Single ejaculate of four buffalo bulls (Nili-Ravi) was collected at weekly intervals for 5 weeks. Ejaculates of acceptable motility (&ge;70%) were pooled and semen was diluted by tris citric acid extender (TCAE; 300 mosm/kg osmotic pressure and ~7.0 pH) having 4 levels of L-cysteine (0.0, 2.0, 5.0 and 8.0 mM). Semen was equilibrated at 4˚C and packaged in 0.5 mL French straws with 20&times;106 spermatozoa/ straw and then frozen in liquid nitrogen. L-cysteine inclusion levels had non-significant (P&gt; 0.05) effect on spermatozoa motility, viability, NAR, PMI and on lipid peroxidation. However, DNA damage in spermatozoa was significantly higher (P&lt;0.05) in 8.0 mM L-cysteine. It was concluded that L-cysteine (2.0 and 5.0 mM) addition to cryopreserved semen had no significant effect on post thaw semen characteristics, except at 8 mM which adversely affected DNA integrity. This may be due to its toxicity at this concentration.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-42"
}]