[{
  "type": "article-journal",
  "title": "MOLECULAR CHARACTERIZATION OF THE σC-ENCODING GENE FROM NOVEL DUCK REOVIRUS",
  "author": [
    {
      "family": "Yun",
      "given": ""
    },
    {
      "family": "Chen",
      "given": ""
    },
    {
      "family": "Hua",
      "given": ""
    },
    {
      "family": "Yu",
      "given": ""
    },
    {
      "family": "Chen",
      "given": ""
    },
    {
      "family": "Ni",
      "given": ""
    },
    {
      "family": "Ye",
      "given": ""
    },
    {
      "family": "Zhang",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2015]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "25",
  "issue": "3",
  "page": "33-41",
  "DOI": "NA",
  "abstract": "<p>We cloned and sequenced the &sigma;C-encoding gene of a novel duck reovirus (N-DRV) isolate ZJ00M. The &sigma;C gene is 966 bp long and is encoded by the third ORF of the S1 segment, a small protein of 321 amino acid residues (approximately 34.1 kDa). Expressed &sigma;C fusion protein in E. coli showed good antigenicity and immunogenicity in a western blotting assay and indirect immunofluorescence assays (IFA). The comparative sequence analyses indicated that N-DRV &sigma;C shared high identity levels with ARV species (classical MDRV and ARV) and a lower degree of identity with other orthoreovirus counterparts, but the sequence identities were no more than 45 %. As observed in classical MDRV and ARV &sigma;C protein sequences, some sequence motifs, conserved sequence motifs and/or functional domains were identified in the sequence of N-DRV &sigma;C. The phylogenetic analysis of &sigma;C/&sigma;1 sequence revealed that N-DRV was different fromother orthoreovirus species groups but remained classified into the avian orthoreoviruses along with other ARVs, suggesting that N-DRV and classical MDRVs constitute a separate geno group relative to ARV. These results are important for future studies examining the structure, function and immunogenicity of N-DRV &sigma;C.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-306"
}]