[{
  "type": "article-journal",
  "title": "NEUROGENIN3 REGULATES SOHLH1 DURING SPERMATOGNESIS IN MICE",
  "author": [
    {
      "family": "Guo",
      "given": ""
    },
    {
      "family": "Li",
      "given": ""
    },
    {
      "family": "Du",
      "given": ""
    },
    {
      "family": "Li",
      "given": ""
    },
    {
      "family": "Yang",
      "given": ""
    },
    {
      "family": "Dong",
      "given": ""
    },
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Zheng",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2015]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "25",
  "issue": "5",
  "page": "1303-1310",
  "DOI": "NA",
  "abstract": "<p>Spermatogonial stem cells (SSCs) are tissue-specific stem cells in testis, and differentiation of SSCs contributes to spermatogenesis and male fertility. However, little is known about factors regulating spermatogonial differentiation.&nbsp;<em>Neurogenin3</em>&nbsp;(<em>Ngn3</em>), a class B basic helix-loop-helix (bHLH) transcription factor, recognized for its role in promoting the development of neurons in the hypothalamus, specifically expresses in undifferentiated spermatogonia and is a critical differentiation factor of mammalian stem and progenitor spermatogonia.&nbsp;<em>Sohlh1</em>&nbsp;(spermatogenesis and oogenesis specific basic helix-loop-helix 1) is regarded as a key factor in spermatogenesis, which promotes the expression of&nbsp;<em>Kit</em>, the marker of differentiated spermatogonia. In this study, we firstly confirmed that the expressionlevelof<em>Ngn3</em>&nbsp;is decreased in&nbsp;<em>S</em><em>ohlh1</em><em>-/-</em>&nbsp;mice by real-time PCR and western blot, and revealed the associationbetween<em>N</em><em>gn3</em>and&nbsp;<em>S</em><em>ohlh1</em>. We then showed that&nbsp;<em>N</em><em>gn3</em>&nbsp;could bind to the promoter of&nbsp;<em>S</em><em>ohlh1</em>andincrease the transcription of&nbsp;<em>S</em><em>ohlh1</em>&nbsp;by ChIP and luciferase reporter gene assay. Lastly, we demonstrated that the protein of&nbsp;<em>N</em><em>gn3</em>&nbsp;and&nbsp;<em>S</em><em>ohlh1</em>&nbsp;could bind together in wild type testis by immune precipitation. In conclusion, our study showed the association between&nbsp;<em>Ngn3</em>&nbsp;and&nbsp;<em>S</em><em>ohlh1</em>&nbsp;for the first time.&nbsp;<em>Ngn3</em>&nbsp;may regulate spermatogenesis by binding with&nbsp;<em>S</em><em>ohlh1</em>, which may be the direct target of&nbsp;<em>Ngn3</em> in differentiation of SSC.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-178"
}]