[{
  "type": "article-journal",
  "title": "EVALUATION OF DNA EXTRACTION METHOD FROM FORMALIN PRESERVED SKIN SAMPLES OF PANTHERA PARDUS FOR MOLECULAR GENETIC ASSESSMENT",
  "author": [
    {
      "family": "Bibi",
      "given": ""
    },
    {
      "family": "Rehman",
      "given": ""
    },
    {
      "family": "Minhas",
      "given": ""
    },
    {
      "family": "Janjua",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2015]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "25",
  "issue": "4",
  "page": "1196-1199",
  "DOI": "NA",
  "abstract": "<p>Five different protocols,<em>&nbsp;viz.&nbsp;</em>Andleeb&nbsp;<em>et al.</em>&nbsp;(2012), Shrivastava&nbsp;<em>et al</em>. (2012), Phenol chloroform DNA extraction method, Roboscreen Kit method and Phenol chloroform triton method, were tested for DNA extraction from skin samples of Leopard (<em>Panthera pardus)&nbsp;</em>preserved in formalin over a period of two to five months. Among these procedures, the Phenol chloroform triton method was proved to be the most efficient protocol with 83% extraction rate and revealed 100% amplification of FCA026 and FCA043 loci. Keeping in views the extraction and amplification efficiency, Phenol chloroform triton method is recommended for molecular studies of formalin preserved skin samples of&nbsp;<em>Panthera pardus</em>.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-161"
}]