[{
  "type": "article-journal",
  "title": "TERATOGENIC AND EMBRYO-TOXIC EFFECTS OF LATEX AND LEAVES METHANOL EXTRACT OF EUPHORBIA HELIOSCOPIA L. IN MICE AND CHICKEN EMBRYOS",
  "author": [
    {
      "family": "Saleem",
      "given": ""
    },
    {
      "family": "Ahmad",
      "given": ""
    },
    {
      "family": "Ahmad",
      "given": ""
    },
    {
      "family": "Hussain",
      "given": ""
    },
    {
      "family": "Bukhari",
      "given": ""
    },
    {
      "family": "Anjum",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2014]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "24",
  "issue": "2",
  "page": "450-454",
  "DOI": "NA",
  "abstract": "<p><em>Euphorbia helioscopia&nbsp;</em>L. has been investigated for antibacterial, antifungal, antiviral and vasodepressor activities. The study was designed to probe into the reproductive toxicity (teratogenicity) potential of&nbsp;<em>Euphorbia helioscopia&nbsp;</em>L. in albino mice. Toxicity of latex and methanol extract of leaves (10, 50, 80, 100 and 120 &micro;g/200 &micro;L) was evaluated in white leghorn chicken embryos (5th day) using chorioallantoic membrane route (n = 30 for each experiment i.e. latex &amp; extract, or 5 per dose and 5 as control group). Teratogenic potential of latex (n= 5) and extract (100, 300, 500, 700, 1000, 1500 and 2000 mg/kg body weight, n= 40, or 5 per concentration and 5 as control group) was evaluated in pregnant albino mice on 7th day of gestation using intra-peritoneal route. Both latex and leave extract checked in chicken embryos were safe. LD50 calculated with TSK software was 1397 mg/kg body weight for methanol extract of leaves observed in albino mice. The rest of the experimental concentrations were non-lethal in mice without any observable malformation. It is concluded that the concentration (administered parentally) less than LD50 is devoid of teratogenic effects in pregnant female mice and chicken embryos.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-64"
}]