[{
  "type": "article-journal",
  "title": "ANTIOXIDANT PROPERTIES OF LACTOBACILLUS AND ITS PROTECTING EFFECTS TO OXIDATIVE STRESS CACO-2 CELLS",
  "author": [
    {
      "family": "Wu",
      "given": ""
    },
    {
      "family": "Sun",
      "given": ""
    },
    {
      "family": "Zhang",
      "given": ""
    },
    {
      "family": "Xin",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2014]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "24",
  "issue": "6",
  "page": "1766-1771",
  "DOI": "NA",
  "abstract": "<p>This study was designed to analyse the antioxidant capacity of lactic acid bacteria (LAB) in order to&nbsp;<a id=\"OLE_LINK55\" name=\"OLE_LINK55\"></a><a id=\"OLE_LINK54\" name=\"OLE_LINK54\"></a>provide characteristic parameters and theoretical basis for its further utilization as a natural antioxidant. Further experiment was carried out to study its protection mechanism through oxidative stress Caco-2 cell model in vitro. For this purpose, antioxidative activity of cells and different cellular fractions&nbsp;<em>of&nbsp;<a id=\"OLE_LINK19\" name=\"OLE_LINK19\"></a><a id=\"OLE_LINK18\" name=\"OLE_LINK18\"></a>Lactobacillus delbrueckii</em>&nbsp;(ATCC7830) was evaluated by scavenging capacity of superoxide anion free radicals (SAFR)，reducing power and total antioxidant capacity assay. Low dose of H2O2 (100&mu;mol/L) was used to establish oxidative damaged model on Caco-2 cells, the bacteria&rsquo;s protecting effect on the damaged cells was further researched. The results showed that extracellular secretions of&nbsp;<em>Lactobacillus delbrueckii&nbsp;</em>has the strongest antioxidant activity, following by cells, and cells can clear superoxide radicals. The ability to scavenge SAFR of this LAB was (42.4&plusmn;0.32) % (cell lysates, the highest), the reducing power was (3.01&plusmn;0.20, cell extracellular secretions, the highest), and the total antioxidant ability was (4.32&plusmn;0.36, cell extracellular secretions, the highest). The above antioxidative character made this LAB possible to protect Caco-2 cells being damaged by low dose of H2O2. Further analysis showed that the total antioxidant capacity (T-AOC) of culture supernatant and lysate were significantly increased compared with the negative control group and the oxidative stress group (<em>p</em>&lt;0.01). Also increased the anti-superoxide anion (O2-) free radicals (<em>p</em>&lt;0.01) level. All the results indicate that<a id=\"OLE_LINK25\" name=\"OLE_LINK25\"></a><a id=\"OLE_LINK24\" name=\"OLE_LINK24\"></a>&nbsp;different fractions from lactic acid bacteria have various capabilities on reducing power, scavenging superoxide anion free radicals ability and total antioxidant capacity. Extracellular secretions have the most obvious antioxidant character.&nbsp;<em>Lactobacillus delbrueckii</em> could protect Caco-2 cells under oxidative stress by increasing its antioxidative function in vitro.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-253"
}]