[{
  "type": "article-journal",
  "title": "IN-OVULE EMBRYO CULTURE OF Thermopsis turcica",
  "author": [
    {
      "family": "Tekdal",
      "given": ""
    },
    {
      "family": "Cetiner",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2014]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "24",
  "issue": "6",
  "page": "1673-1679",
  "DOI": "NA",
  "abstract": "<p>The possibility of micropropagation for the endangered&nbsp;<em>Thermopsis turcica&nbsp;</em>wasinvestigated through an fruit ovule-embryo culture. This technique prevents embryo degradation at the early development stage, shortens breeding time, and enables efficient propagation of many plants from a single embryo, thus avoiding for breaking seed dormancy. Although a number of efficient propagation protocols of&nbsp;<em>T. turcica&nbsp;</em>has been reported for multiple shoot induction and plantlet regeneration from the seeds of&nbsp;<em>T. turcica</em>, to date no publication exists on any protocol on embryo culture of&nbsp;<em>T. turcica</em>. This paper reports on the optimisation of protocols for culturing of&nbsp;<em>T.</em>&nbsp;<em>turcica</em>&nbsp;fruit embryos. The protocol for embryo culture used in this research consisted of cracking the fruit, pitting it to remove the seed, excising the ovule-embryo from the seed, placing it on the medium modified from established Fabaceae embryo tissue culture media, extracting embryos from ovular integuments, and culturing. In the present study, extracted ovule-embryos were cultured on a Murashige and Skoog&rsquo;s medium supplemented with Zeatin (0.5 mg L-1), Giberellic acid &nbsp;(0.5 mg L-1), and Indole-3-acetic acid (0.2 mg L-1). Excised embryos from the ovular integuments were maintained on Giberellic acid-free MS medium. During culturing, cotyledons began to arise and grow plantlets. Regenerated plantles were rooted on a MS medium with Indole-3-butyric acid (2.0 mg L-1). These results show that embryo culture technique can be an alternative ex-situ conservation technique for&nbsp;<em>in vitro</em>&nbsp;propagation of the threatened rare plant species&nbsp;<em>T. turcica</em>.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-240"
}]