[{
  "type": "article-journal",
  "title": "SIMPLE AND RAPID CULTIVATION OF TRYPANOSOMA DANILEWSKYI STRAIN FCC-1 IN MONOPHASIC LIQUID CULTURE MEDIUM",
  "author": [
    {
      "family": "Ahmed",
      "given": ""
    },
    {
      "family": "Ahmed",
      "given": ""
    },
    {
      "family": "Khan",
      "given": ""
    },
    {
      "family": "Ali",
      "given": ""
    },
    {
      "family": "Ollevier",
      "given": ""
    },
    {
      "family": "Ray",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2014]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "24",
  "issue": "1",
  "page": "149-153",
  "DOI": "NA",
  "abstract": "<p><em>Trypanosoma danilewskyi</em>&nbsp;strain F Cc-1 was isolated from the blood of a laboratory infected juvenile common carp.&nbsp; Trypanosomes (blood stream forms) were first adapted to culture conditions in Tobie&rsquo;s medium.&nbsp; Ten sterilized glass culture tubes each containing 2 ml of Tobie&rsquo;s medium solidified in slants and covered with 1 ml Locke-Krebs overlay, were inoculated with 0.1 ml of infected blood containing 1x 105 live trypanosomes and incubated at 20&ordm; C.&nbsp; Tobie's medium supported growth and trypanosomes were well adapted to culture conditions because small colonies were seen in 6th and 7th subculture.&nbsp; The adapted trypanosomes were re-inoculated in a monophasic liquid culture medium (L4 NHS).&nbsp; Ten sterilized caped glass culture tubes, each containing 3 ml of medium, were inoculated with 0.1 ml culture containing 3 x 105 flagellates and incubated at 20&ordm; C.&nbsp; Trypanosome propagation / multiplication were examined on day 3 p.i. and daily later on and their number ml-1 was estimated by the Rapid Matching Method.&nbsp; Trypanosomes growth obtained in L4 NHS culture medium on day 7-8 was 5.0 &plusmn; 0.56 x 106 live trypanosomes ml-1.&nbsp; The trypanosome number significantly (P&lt;0.05) increased from day 3 to 8 as compared to day 1.&nbsp; Flagellate numbers remained high until day 10 but decreased from day 12-15 p.i.&nbsp; L4 NHS, mono-phasic culture medium supported growth of&nbsp;<em>T. danilewskyi</em> strain FCc-1 (culture forms) significantly high (P&lt;0.01) at 20&ordm; C and found the best for rapid fish trypanosome culture.&nbsp;</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-22"
}]