[{
  "type": "article-journal",
  "title": "VIRTUAL SCREENING AND BIOLOGICAL EVALUATION OF AVIAN ERYTHROBLATOSIS VIRUS E26 ONCOGENE (ETS-1) INHIBITOR",
  "author": [
    {
      "family": "Mehreen",
      "given": ""
    },
    {
      "family": "Javeed",
      "given": ""
    },
    {
      "family": "Ashraf",
      "given": ""
    },
    {
      "family": "Riaz",
      "given": ""
    },
    {
      "family": "Hussain",
      "given": ""
    },
    {
      "family": "Shakir",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2014]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "24",
  "issue": "5",
  "page": "1334-1341",
  "DOI": "NA",
  "abstract": "<p>This study was designed to identify and characterize novel inhibitor of Ets-1 by using virtual screening approach combined with biological evaluation of anti-angiogenic ability of compound. Virtual screening of Indian plant anticancer compound database was performed against phosphorylation site (Threonine-38) of Ets-1 by Auto Dock 4.02. A compound with lowest energy score (-4.28kcal/mol) was selected. Biological activity of compound obtained from virtual screening was further evaluated by performing chick chorioallantoic membrane (CAM) assay. Different concentrations (0.5 %, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 6%, and 12%) of aqueous extract of saffron plant containing screened compound (Picrocrocin) were applied to CAMs and scanning probe image processor was used for quantification of membrane vasculature. Results showed that application of 2% and 3% concentration of extract showed reduction in diameter of blood vessels, decrease in abbot curve area and reduction in surface roughness parameters. This study revealed a potential inhibitor of Ets-1 and anti-cancer mechanism of saffron.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-194"
}]