[{
  "type": "article-journal",
  "title": "THIOGLYCOL IN EXTENDER IMPROVES THE POST-THAW QUALITY OF BUFFALO (BUBALUS BUBALIS) BULL SPERMATOZOA",
  "author": [
    {
      "family": "Ansari",
      "given": ""
    },
    {
      "family": "Rakha",
      "given": ""
    },
    {
      "family": "Andrabi",
      "given": ""
    },
    {
      "family": "Ullah",
      "given": ""
    },
    {
      "family": "Iqbal",
      "given": ""
    },
    {
      "family": "Holt",
      "given": ""
    },
    {
      "family": "Akhter",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2014]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "24",
  "issue": "4",
  "page": "1256-1259",
  "DOI": "NA",
  "abstract": "<p>This study was designed to evaluate the effect of thioglycol in extender on post-thaw quality of Nili-Ravi buffalo (<em>Bubalus bubalis</em>) bull spermatozoa. Semen from three buffalo bulls was collected at weekly intervals (three replicates; two ejaculates per bull per replicate) and qualifying ejaculates (n=18) were cryopreserved in extenders containing thioglycol at 0.0 or 0.5 or 1.0mM. Sperm motility (%; visually), plasma membrane integrity, (%;supravital hypo-osmotic swelling test), viability (%;dual staining technique using Trypan blue and Giemsa stains) and DNA integrity (%;acridine orange test) was assessed at 0, 2 and 4 hour post-thaw. Sperm motility, plasma membrane integrity and viability of buffalo bull spermatozoa were improved in a dose dependent manner with the supplementation of thioglycol at 0.5 mM and 1.0mM compared control. Sperm DNA integrity was similar (P&gt;0.05) in extenders containing 0.5 mM and 1 mM thioglycol that remained higher (<em>P</em> &lt; 0.05) than the values of DNA integrity in control. It is concluded that 1.0mM thioglycol in extender improved the motility, plasma membrane integrity, viability and DNA integrity of buffalo bull spermatozoa.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-180"
}]