[{
  "type": "article-journal",
  "title": "NANO-GOLD PARTICLES MEDIATED DETECTION OF NS1; AN EARLY DIAGNOSTIC MARKER OF DENGUE VIRUS INFECTION",
  "author": [
    {
      "family": "Hussain",
      "given": ""
    },
    {
      "family": "Rehman",
      "given": ""
    },
    {
      "family": "Aslam",
      "given": ""
    },
    {
      "family": "Javed",
      "given": ""
    },
    {
      "family": "Abbas",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2014]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "24",
  "issue": "4",
  "page": "1110-1115",
  "DOI": "NA",
  "abstract": "<p>In this study, an immunoassay is developed for qualitative determination of NS1 antigen of dengue virus in serum using nano-gold particles. This diagnostic device comprises of four components such as sample pad, conjugation pad, nitrocellulose membrane and absorbent pad. Anti-NS1 antibodies and rabbit anti-mouse IgG antibodies were coated on nitrocellulose membrane as a test and control line respectively. Assay device was evaluated by purified NS1 and prior ELISA confirmed NS1 positive and negative samples. The diagnostic device showed promising results to detect NS1.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-160"
}]