[{
  "type": "article-journal",
  "title": "DIFFERENTIAL EXPRESSION AND BIOINFORMATICS ANALYSIS OF MIR-184 IN PREGNANT AND NON-PREGNANT GOAT OVARIES",
  "author": [
    {
      "family": "Zhang",
      "given": ""
    },
    {
      "family": "Ding",
      "given": ""
    },
    {
      "family": "Ling",
      "given": ""
    },
    {
      "family": "Li",
      "given": ""
    },
    {
      "family": "Zhang",
      "given": ""
    },
    {
      "family": "Zhang",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2013]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "23",
  "issue": "6",
  "page": "1545-1552",
  "DOI": "NA",
  "abstract": "<p>Ovarian follicular development and hormone secretion are complex and coordinated biological processes which alter during pregnancy. Ovarian function is tightly regulated by a multitude of genes, and may also be regulated by specific miRNAs. It is necessary to identify the differentially expressed miRNAs in the ovaries of pregnant and non-pregnant mammals, in order to fully understand the role of miRNA-mediated post-transcriptional regulation in mammalian reproduction. In an effort to uncover miR-184 importance in goats, we examined the differential expression pattern in the ovaries of 5 pregnant and 5 non-pregnant goats and gene ontology (GO) term enrichment of predicted target genes to determine the global biological functions of miR-184. Quantitative real-time PCR (q-PCR) analysis showed that miR-184 was expressed in the ovaries of pregnant goats, but was not expressed in the ovaries of non-pregnant goats. Moreover, thirty-eight target genes of miR-184 were predicted and 10 functional categories of GO terms were significantly overrepresented among the predicted miR-184 target genes. These categories can be broadly defined as regulation of development process and cellular biosynthetic process. Our results demonstrated that miR-184 was involved in various biological processes including pregnancy, developmental and cellular biosynthetic processes. Stephens and Moley 2009) . In the non-pregnant phase,</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-611"
}]