[{
  "type": "article-journal",
  "title": "A NOVEL THROMBIN-LIKE GENE OBTAINED FROM CHINESE GLOYDIUS SHEDAOENSIS SHEDAOENSIS",
  "author": [
    {
      "family": "Guo",
      "given": ""
    },
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Zheng",
      "given": ""
    },
    {
      "family": "Sun",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2013]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "23",
  "issue": "5",
  "page": "1398-1406",
  "DOI": "NA",
  "abstract": "<p>The snake of&nbsp;<em>Gloydius shedaoensis shedaoensis</em>&nbsp;(GSS) is the particular and sole snake species from Shedao island in Dalian, China. A novel gene named as GSSG-TLE was cloned from GSSG by PCR using GSSG-cDNA library plasmid as template. The GSSG-TLE cDNA was sequenced and encompasses an open reading frame of 786 bp in length and encoding a protein of 262 amino acid residues. The&nbsp;<a id=\"OLE_LINK46\" name=\"OLE_LINK46\">GSSG-TLE</a>&nbsp;gene has a base composition of 14 Ala, 12 Cys, 14 Asp, 13 Glu, 10 Phe, 19 Gly, 7 His, 20 Ile, 15 Lys, 25 Leu, 4 Met, 12 Asn, 19 Pro, 7 Gln, 12 Arg, 20 Ser, 14 Thr, 13 Val, 5 Trp and 7 Tyr. GSSG-TLE includes a signal peptide of 18 amino acids, a proposed propeptide of 6 amino acids and a matured peptide of 238 amino acids. It also contains 12 Cys which form 6 disulfide bridges and has three conserved catalytically active sites of His67, Asp112 and Ser202. Sequence comparison revealed that GSSG-TLE amino acids sequence shared highly identity with other snake venom<a id=\"OLE_LINK45\" name=\"OLE_LINK45\">&nbsp;thrombin-like enzyme</a>s.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-529"
}]