[{
  "type": "article-journal",
  "title": "MOLECULAR CLONING AND ANALYSIS OF A CONSTANS HOMOLOG FROM NICOTIANA TABACUM",
  "author": [
    {
      "family": "Lu",
      "given": ""
    },
    {
      "family": "Liu",
      "given": ""
    },
    {
      "family": "Sun",
      "given": ""
    },
    {
      "family": "Mu",
      "given": ""
    },
    {
      "family": "Ren",
      "given": ""
    },
    {
      "family": "Zhang",
      "given": ""
    },
    {
      "family": "Sciences",
      "given": ""
    },
    {
      "family": "Beijing",
      "given": ""
    },
    {
      "family": "China",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2013]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "23",
  "issue": "5",
  "page": "1360-1365",
  "DOI": "NA",
  "abstract": "<p>Plant development is largely controlled by day length. In&nbsp;<em>Arabidopsis thaliana</em>, the&nbsp;<em>CONSTANS&nbsp;</em>(<em>CO</em>) gene plays a pivotal role in the photoperiodic pathway controlling floral transition. In this research, we employed&nbsp;<em>Nicotiana tabacum</em>&nbsp;<em>cv. Kutsaga Mammoth 10</em>&nbsp;as experimental materials and designed hetrologous primers based on the&nbsp;<em>S. tuberosum</em>&nbsp;CO gene sequence and tobacco expressed sequence tags (ESTs). By using the approach of RT&ndash;PCR combined with rapid amplification of cDNA ends (RACE) technology, a novel cDNA encoding a CO homolog named<em>&nbsp;NtCO1&nbsp;</em>(GenBank accession number, JN022535.1) was cloned. The full-length cDNA sequence isolated was 1493 bp with an open reading frame (ORF) of 1211 bp, encoding a protein of 403 amino acids. The predicted NtCO1 protein contains two B-box-type zinc fingers and a CCT domain. Analysis based on amino acid sequence alignment showed that NtCO1 shares high identity with StCO (86.5%) from&nbsp;<em>Solanum tuberosum</em>&nbsp;, AtCO (50%) from&nbsp;<em>A. thaliana</em>&nbsp;and Hd1 (43.7%) from&nbsp;<em>Oryza sativ</em>a. Semi-quantitative RT&ndash;PCR analysis showed that&nbsp;<em>NtCO1</em>&nbsp;was expressed specifically and strongly in leaf tissues but weakly in stem and root tissues.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-523"
}]