[{
  "type": "article-journal",
  "title": "AUGMENTATION OF BIOLOGICAL TITER OF FOOT AND MOUTH DISEASE VIRUS IN IN VITRO CULTURE",
  "author": [
    {
      "family": "Akram",
      "given": ""
    },
    {
      "family": "Muhammad",
      "given": ""
    },
    {
      "family": "Rabbani",
      "given": ""
    },
    {
      "family": "Nazir",
      "given": ""
    },
    {
      "family": "Nawaz",
      "given": ""
    },
    {
      "family": "Hanif",
      "given": ""
    },
    {
      "family": "Shakoor",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2013]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "23",
  "issue": "3",
  "page": "771-774",
  "DOI": "NA",
  "abstract": "<p>Foot and Mouth Disease (FMD) virus grew well on BHK-21 cell line. The virus showed poor tissue culture infective dose 50 (TCID50) that was log 103.2&plusmn;0.2 in BHK-21 cell line having Glasgow Minimal Essential Medium (GMEM) without Fetal Calf Serum (FCS). Addition of one per cent FCS in the medium @ one percent increased log 107.1&plusmn;0.2 units of virus TCID50. Incubation temperature of 350 C and 370 C supported the multiplication and maintenance of BHK-21 cells and yielded log106.6&plusmn;0.1 and log107.0&plusmn;0.2units of virus TCID50, respectively. Each serotype of FMD virus showed log106.29&plusmn;0.07 units of virus TCID50 in the stationary monolayer of BHK-21 cells in roux flask (75cm 2), log107.66&plusmn; 0.02 units of virus TCID50 in roller bottles (490cm2) and log108.34 &plusmn; 0.07 units of virus TCID50 on 0.2 g of micro-carriers suspending in 200 ml of the growth medium in stirring bottle. The infectivity titer/TCID50 of each of the virus serotypes was significantly higher in roller bottles than that achieved in roux flasks (p&lt;0.05) and was significantly higher in stirring bottle containing micro-carriers suspending in the growth medium than that of harvested in roller bottle (p&lt;0.05). It is concluded that the infectivity titer of the virus is directly proportional to number of BHK-21 cells in the culture system.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-315"
}]