[{
  "type": "article-journal",
  "title": "ESTABLISHMENT OF THE HONEY CROP (STEVIA REBAUDIANA) I N HOT SEMI ARID CLIMATE",
  "author": [
    {
      "family": "Javad",
      "given": ""
    },
    {
      "family": "Naz",
      "given": ""
    },
    {
      "family": "Ilyas",
      "given": ""
    },
    {
      "family": "Mateen",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2013]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "23",
  "issue": "1",
  "page": "108-113",
  "DOI": "NA",
  "abstract": "<p>Stevia rebaudiana is native plant of tropical climate of Brazil and Paraguay. Its leaves produce a sweetener- stevioside which is 150-300 times sweeter than cane sugar. An attempt is made to grow this plant in hot semi arid climate of Lahore region of Pakistan through tissue culture technique. A completely randomized design was used for the experiment with three replicates. The data was analyzed by applying one way ANOVA and the treatments&rsquo; means were compared for significance by Duncan&rsquo;s New Multiple Range (DMR) test at 0.05% P. MS media supplemented with different concentrations of BAP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l), kinetin (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l) and combination of BAP and kinetin were used and BAP 1 mg/l proved to be the better micropropagating medium for Stevia rebaudiana as compared to other media. Whilst rooting of micro shoots of the Stevia gave the best response in MS+ IBA 0.4 mg/l. then these mericlones were hardened in green house in different soil mixtures among which the best mixture was sand, clay and vermicompost in the ratio of 1:1:1 with 85% survival rate. These semi hardened plants were then shifted to larger pots and open field of hot semi arid climate of Lahore and developed successfully.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-117"
}]