[{
  "type": "article-journal",
  "title": "ANTIBACTERIAL ACTIVITY OF A TRYPSIN-CHYMOTRYPSIN-ELASTASE INHIBITOR ISOLATED FROM LAVATERA CASHMERIANA CAMB.SEEDS",
  "author": [
    {
      "family": "Rakashanda",
      "given": ""
    },
    {
      "family": "Ishaq",
      "given": ""
    },
    {
      "family": "Masood",
      "given": ""
    },
    {
      "family": "Amin",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2012]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "22",
  "issue": "4",
  "page": "983-986",
  "DOI": "NA",
  "abstract": "<p>Protease inhibitors were extracted from the seeds of&nbsp;<em>Lavatera cashmeriana</em>&nbsp;Cambby ammonium sulphate precipitation and purified by chromatography on DEAE-cellulose and Sephadex G-100 column. The bound protein eluted into four major peaks which we named as LC-pi I, II, III and IV, all showing strong anti protease activity against trypsin, chymotrypsin and elastase. LC-pi I was screened for antibacterial activity against&nbsp;<em>Klebsiella pnuemoniae, &nbsp;Escherichia coli</em>&nbsp;and&nbsp;<em>Pseudomonas aeruginosa</em>. The protease inhibitor showed strong antibacterial activity against&nbsp;<em>Klebsiella pnuemoniae &nbsp;</em>and&nbsp;<em>Pseudomonas aeruginosa</em>&nbsp;but was less active against&nbsp;<em>&nbsp;Escherichia&nbsp; coli</em>.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-427"
}]