[{
  "type": "article-journal",
  "title": "EFFECT OF ORGANOPHOSPHATE PESTICIDE MALATHION ON IN-VITRO OOCYTE MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT OF NILI RAVI BUFFALO (BUBALUS BUBALIS)",
  "author": [
    {
      "family": "Aziz",
      "given": ""
    },
    {
      "family": "Mehboob",
      "given": ""
    },
    {
      "family": "Jalali",
      "given": ""
    },
    {
      "family": "Shami",
      "given": ""
    },
    {
      "family": "Qureshi",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2012]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "22",
  "issue": "3",
  "page": "147-152",
  "DOI": "NA",
  "abstract": "<p>The present study evaluated in-vitro toxicity of organophosphate insecticide, malathion on nuclear maturation, fertilization and subsequent embryo development of the oocytes obtained from abattoir ovaries of Nili-Ravi Buffalo (Bubalus bubalis). The cumulus-oocyte complexes (COCs) in culture medium were exposed to four different regimens of malathion, 1.56 &micro;mol, 3.12 &micro;mol, 6.25 &micro;mol and 12.5 &micro;mol. Results demonstrated dose dependent significant difference (P &lt; 0.05) in nuclear competence, oocyte maturation and degeneration. Cumulus expansion showed significant increase (P &lt; 0.05) while oocyte diameter increased non significantly (P &gt; 0.05). The COCs that attained nuclear maturation (MII) and were subjected to IVF and, subsequent embryonic development also showed dose dependent non-significant (P &gt; 0.05) decrease in fertilization and embryonic development, however, hatched blastocyst formation was significantly compromised (P &lt; 0.05). The blastocyst development with inner cell mass was again non- significantly different (P &gt; 0.05). The study concludes that malathion may effect quality of preimplantation embryos developed in-vitro from immature oocytes pre-exposed to the pesticide.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-3006"
}]