[{
  "type": "article-journal",
  "title": "COMPARATIVE GROWTH PROMOTING EFFICACY OF FETAL CALF SERUM (FCS) FOR BABY BAMSTER KIDNEY-21 (BHK-21) CELL LINE",
  "author": [
    {
      "family": "Rahman",
      "given": ""
    },
    {
      "family": "Ahmad",
      "given": ""
    },
    {
      "family": "Bhatti",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2006]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "16",
  "issue": "2",
  "page": "NA",
  "DOI": "NA",
  "abstract": "<p>A study was conducted to evaluate the growth promoting effect of FCS and its proper dilution in cell culture media for persistent cultivation and maintenance of BHK-21 cell culture, by comparing it with other animal sera. Different animal sera of different species as cattle calf serum , Allanto-amniotic fluid , Sheep serum , Poultry serum and Goat serum @ 10% were added to cell culture medium (M 199, Biomedicals; USA). A fast and reliable technique, MTT (3 {4, 5-Dimethyzol-2-Yl} 2, 5-Diphenyl Tetrazolium Bromide) Assay was employed to measure the cell densities in small amount of cell culture media by measuring OD values under 570nm, with 630nm as a reference read out. It was concluded that FCS is the best growth promoting agent for BHK-21 cell line and 5-7.5 % concentration has the desired effect of persistent cultivation and maintenance of BHK-21 cell line.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=2006-JAPS-215"
}]