[{
  "type": "article-journal",
  "title": "COMPARISON OF ANALYTICAL SENSITIVITY AND SPECIFICITY OF REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (RT-LAMP) AND REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR) FOR DETECTION OF LOCAL PESTIVIRUS A",
  "author": [
    {
      "family": "Hammad-ur-Rehman1",
      "given": ""
    },
    {
      "family": "Ahmad1",
      "given": ""
    },
    {
      "family": "Rabbani",
      "given": ""
    },
    {
      "family": "Tipu3­",
      "given": ""
    }
  ],
  "issued": {
    "date-parts": [[2020]]
  },
  "container-title": "Journal of Animal and Plant Sciences",
  "ISSN": "1018-7081",
  "volume": "30",
  "issue": "1",
  "page": "50-57",
  "DOI": "https://doi.org/10.36899/JAPS.2020.1.0006",
  "abstract": "<p>The main aim of this study was to compare reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription polymerase chain reaction (RT-PCR) in order to determine their analytical sensitivity and specificity using local characterized Pestivirus A. In order to compare the sensitivity of RT-LAMP and RT-PCR, serial 10 fold dilutions containing 9.91 x 1010 to 9.91 x 10-2 copies of cDNA of Pestivirus A, were prepared and tested. RT-LAMP proved more sensitive and was able to detect 9.91x100 copies of cDNA compared to RT-PCR (9.91x101). Both RT-LAMP and RT-PCR were found equally specific as no cross reaction with bovine herpes virus was observed.&nbsp; Present study showed that RT-LAMP assay is highly sensitive, specific, rapid and can be used as an alternative to conventional RT-PCR, for the detection of Pestivirus A.</p>",
  "publisher": "Pakistan Agricultural Scientists Forum",
  "URL": "https://thejaps.org.pk/AbstractView.aspx?mid=06-IBC-19-017"
}]