The rapid growth of the aquaculture industry has necessitated the exploration of novel feed additives to enhance fish production performance and health status. This study evaluates the effects of pyrroloquinoline quinone (PQQ) on the production performance, antioxidative capacity, and nonspecific immune response of Plecoglossusaltivelis, with a focus on elucidating its functional mechanisms. Through precise control of PQQ supplementation dosages(0.2, 0.4, and 0.6 mg/kg in the diet), the study analyzed its impact on growth metrics, serum biochemical parameters, antioxidant enzyme activities, and intestinal digestive enzyme activities in Plecoglossusaltivelis. Results indicated that PQQ supplementation markedly improved the growth performance of Plecoglossusaltivelis, including final body weight, weight gain rate, specific growth rate, and condition factor (P<0.05). Additionally, PQQ reduced serum triglyceride concentrations (P<0.05) and enhanced antioxidant enzyme activities, including catalase and superoxide dismutase (P<0.05), as well as total antioxidant capacity (P<0.05). Regarding nonspecific immunity, PQQ elevated serum immunoglobulin M levels, lysozyme activity, and complement C3 content (P<0.05). Furthermore, PQQ stimulated intestinal protease activity (P<0.05).These findings demonstrate that PQQ effectively enhances the production performance, antioxidative capacity, and nonspecific immune response of Plecoglossusaltivelis.