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Volume 28, No. (2), 2018 (April)

ADDITIVE EFFECTS OF COCONUT WATER WITH VARIOUS HORMONES ON IN VITRO REGENERATION OF CARNATION (DIANTHUS CARYOPHYLLUS L.)

M. M. Khatun, P. K. Roy, Md. Abdur Razzak

M. M. Khatun1, P. K. Roy2 and Md. Abdur Razzak3*

1Plant Biotechnology Division, National Institute of Biotechnology, Savar, Dhaka, Bangladesh

2Institute of Food and Radiation Biology, Atomic Energy Research Establishment, 3787, Dhaka-1000, Bangladesh

3*Pohang University of Science and Technology, Pohang 790–784, South Korea
Corresponding Author: razzakbtge@gmail.com
Page Number(s): 589-596
Published Online First: April 01, 2018
Publication Date: April 01, 2018

ABSTRACT

Plant needs some essential nutrient as well as growth regulator for their growth and development. In the case of Carnation (Dianthus caryophyllus L.) micro-propagation, large scale production was not possible by using the available traditional media (MS media) and method. In this study we first demonstrated the in vitro effect of coconut water (CW) together with cytokinine hormone on efficient growth of Carnation. The highest percentage of shoot induction was observed when the medium was used with coconut water (cw) at 10% +BAP 1.0 mg/l. In this case multiplication rate of carnation increased more than four times when shoot tip and nodal segment were used as an explant. So our modified media could be useful for Carnation (Dianthus caryophyllus L.) micro propagation and be a powerful media for tissue culture of Carnation. The best Carnation growth was observed in half-strength MS supplemented with 1.0 mg/l NAA for root induction. The survival rate of the transplanted plantlets was 80% in potted soil condition.
Keywords: Micro propagation, Carnation, Media, Coconut water, Growth regulator
Open Access: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).
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