INVESTIGATIONS ON ONOSMA HISPIDUM WALL ROOT EXTRACTS FOR IN-VITRO ANTIDIABETIC, PROLIFERATIVE AND CYTOTOXIC EFFECTS
1M. Asghar, 1M. Islam, *2H. Saeed, 1F. Imtiaz,1B. Saleem, 2Z. Saleem,4S. Qamar and 3M. Iqtedar
1Section of Pharmaceutical Chemistry, University College of Pharmacy, University of the Punjab,
2Section of Pharmacy Practice, University College of Pharmacy, University of the Punjab,
3Department of Biotechnology, Lahore College for Women University,
4Section of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Lahore
Corresponding Author e-mail. email@example.com
The aim of this study was to investigate the impact of various extracts of roots of Onosma hispidum for anti-diabetes, proliferative and cytotoxic effects using in-vitro assays. Phytochemical screening of powder and extracts was done by employing standard methods. Anticancer activity was assessed by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and short term proliferation assay on HepG2 cell line. The antidiabetic potential was determined by using alpha-amylase inhibition assay and non-enzymatic glycosylation of haemoglobin assay. Data suggested that primary (CHO; 68.06 ± 0.19 and Proteins; 9.48 ± 0.27) and secondary metabolites (mg/g); max. Flavonoids (47.55 ± 0.13) in aqueous extract, max. Polyphenols (107.77 ± 0.57) in Pt. ether extract and max. glycosaponins (94.5 ± 0.51) in chloroform extract, exhibited variations among various extracts obtained from roots of Onosma hispidum. Petroleum ether and aqueous extracts exhibited significant impact (p<0.05) on the proliferation of HepG2 cell lines at different time points compared to control, while in case of MTT assay, no significant results(p>0.05) were obtained after overnight incubation. Improved antidiabetic activity was exhibited by petroleum ether extract (70% inhibition) as compared to Acarbose standard using alpha-amylase inhibition assay, with an IC50 of 80µg/ml. In non-enzymatic glycosylation of haemoglobin assay, better potential was shown by chloroform extract with an IC50of 35µg/ml, when compared to a standard - Tocopherol. This study revealed that the roots of Onosma hispidum possess nutritional value and antidiabetic properties, along with significant impact on cell (HepG2) proliferation.
Keywords: Onosma hispidum, In-vitro, Polyphenols, Antidiabetic, Anticancer.