BIOTECHNOLOGICAL METHODS AND LIMITATIONS OF MICROPROPAGATION IN PAPAYA (CARICA PAPAYA L.) PRODUCTION: A REVIEW
Baker Al-Shara1, Rosna Mat Taha*1, and Kamaludin Rashid2
1Institute of Biological Sciences, Faculty of Science, University Malaya, 50603 Kuala Lumpur, Malaysia
2Biology Division, Center for Foundation Studies Building, University of Malaya, 50603 Kuala Lumpur, Malaysia
*Corresponding author: Rosna Mat Taha email: firstname.lastname@example.org”
Carica papaya L. is considered one of the most important fruit, especially in tropical and subtropical regions. It generates flowers continuously throughout the year and has been conventionally cultivated by seeds. This review summarizes the morphological and evolutionary descriptions of Carica papaya L., the protocols used to achieve in vitromicropropagation of Carica papaya using different plant tissue culture techniques, as well as the problems faced by papaya micropropagation. While there are many problems encountered during in vitro propagation of papaya through somatic embryogenesis, this review focuses specifically on contamination, rooting processes and acclimatization. Solving these problems will enable a reproducible protocol to be established. Subsequently, high-quality papaya plants can be produced and strains that are resistant towards various diseases can be generated via molecular studies. Cryopreservation and synthetic seeds technology are also potential methods for large scale production and crop improvement of papaya.
Keyword: Papaya, Somatic embryogenesis, Acclimatization, Cryopreservation, Synthetic seeds.