URINARY BLADDER RELAXANT EFFECT OF ARTEMISIA VULGARIS AND
ASPALATHUS LINEARIS
Arif-ullah Khan1* and A. H. Gilani2
1Department of Pharmacology, Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan
2Natural Products Research Unit, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi-74800, Pakistan
*Corresponding authors e-mail: arif.ullah@riphah.edu.pk, anwarhgilani@yahoo.com
ABSTRACT
In isolated rabbit urinary bladder preparations, Artemisia vulgaris (Av.Cr) crude extract inhibited the contractions produced by carbachol (CCh, 1 µM) and high K+ (80 mM) in a concentration-dependent (0.03-10 mg/mL) manners. The crude extract of Aspalathus linaeris (Al. Cr) relaxed the contractions produced by low K+ (25 mM) at concentration range of 0.3-1.0 mg/mL, having moderate inhibitory effect on contractions produced by K+ (80 mM). Contractions induced by low K+ were partially antagonized by glibenclamide (3 µM). These results indicate that urinary bladder relaxation produced by Artemisia vulgaris extract is mediated via dual, anticholinergic and Ca++ antagonist mechanisms, while that of Aspalathus linaris via dominant KATP channel opening and weak Ca++ channel blockade pathways. Thus the present study reveals the Artemisia vulgaris and Aspslathus linaeris medicinal usefulness in hyperactive bladder disorders
Key words: Artemisia vulgaris, Aspalathus linaris, urinary bladder relaxation.
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