PLASMID PROFILING AND CURING OF MULTIDRUG RESISTANT ESCHERICHIA COLI RECOVERED FROM RETAIL CHICKEN MEAT
M. Gohar1, A. A. Sheikh1, A. A. Anjum1, T. Hussain2, J. Muhammad1, A. Tabbassum1, A. Kanwal1and I. Safdar1
1University Diagnostic Lab, 2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore.
Corresponding author: email@example.com
Confirmed E. coli (n = 25) were analyzed for the presence of plasmids and cured using temperature and sodium dodecyl sulphate (SDS) treatments. Plasmid profiling of the isolates showed that 96 % isolates were harboring plasmids of varying size and number. The average plasmid number among the isolates was 2.28, ranging from 0 – 5 plasmids per isolate and plasmid size varied from 100bp – 12kb which was distributed randomly in these isolates. Statistically no significant correlation (p>0.05) was found between antibiotic resistance and plasmid profiles. Plasmid curing study showed 3.9% and 16.7% curing efficiency with that of temperature and SDS treatment, respectively. Statistical analysis revealed that resistant isolates, treated with either physical or chemical treatments produced significant results (p<0.05) of plasmid curing. Antimicrobial resistance genes might be located on plasmid and statistically no relationship was observed between antibiotic resistance and plasmid profiles.
Key words: Plasmid profiles, plasmid curing, E. coli, antibiotic resistance