The absorption of the minerals produced by the probiotics in ruminants is expected to improve the absorption of the required nutrients. The study used different dietary rations with different percentage of concentrates: forages, different selenium doses, different locations and different strains of lactic acid bacteria as probiotics. The trials were performed with three repetitions using a factorial block (2x2x3x4). Samples were incubated for 48 h using in vitro incubation system. The parameters were pH, the kinetics of gas production, in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), and NH₃-N concentration. The data were analyzed using the ANOVA, followed by the Duncan test. The results showed altered fermentation characteristics, while BioNano-Se at 25 ppm has significantly increased the rumen pH, gas production rate, IVDMD, IVOMD and decreased the total gas production significantly, but did not affect the ammonia concentration. High concentrate rations (R1) produces significantly lower pH (P < 0.05), but higher gas production rate and digestibility (P < 0.05). Higher dose of BioNano-Se significantly decreased gas production rate and ammonia production (P < 0.05). In conclusion, BioNano-Se and probiotics can alter rumen fermentation process.