The experiment was conducted to determine the genetic relationship between polymorphism of lipoprotein lipase(LPL) gene and intramuscular fat content in goat. 337 goats were collected from 6 goat populations including Anhui white goats, Boer goats, Linqu dairy goats, Minshan black fur goats, Jining grey goats and Matou goats. The genetic variations in exon 1,6, and 7 of LPL gene were detected by PCR-SSCP. Genetic effect of LPL gene on intramuscular fat content was analyzed by Anhui White and Boar populations. The results exhibited as follows: one polymorphism site was found in the exon1 of LPL and five genotypes were defined. Besides that, one polymorphism site was found in exon 6 and 7 of LPL gene, respectively, andthree genotypes AA, AB and BB were defined in both of exon 6 and 7. LPL gene increased intramuscular in the order of P1 (exon 1 of LPL): BB>AB>AA, P6 (exon 6 of LPL): AA>AB>BB, and P7 (exon 7 of LPL): BB>AB>AA. These results indicated that LPL gene could be considered as a candidate gene affecting intramuscular fat content in special goat populations.