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Volume 24, No. (6), 2014 (December)
IMMUNOGENIC ACTIVITY OF LIPOPOLYSACCHARIDES FROM PASTEURELLA MULTOCIDA IN RABBITS
A. Ashraf, S. Mahboob, K. Al-Ghanim, T. Huma and M. S. Shah

A. Ashraf2, S. Mahboob1, 2, K. Al-Ghanim1, T. Huma4and M. S. Shah3

1Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11455, Kingdom of Saudi Arabia
2Department of Zoology, GC, University Faisalabad-Pakistan
3Department of Bioinformatics & Biotechnology, GC University Faisalabad, Pakistan
4Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan
Corresponding Author: shahidmahboob60@hotmail.com
DOI: NA
Page Number(s): 1780-1785
Published Online First: December 01, 2014
Publication Date: December 01, 2014
ABSTRACT

Lipopolysaccharides (LPS) were extracted from the whole cell of the causative organism (Pasteurella multocida) and quantified by using the semi-carbazide method. This quantified antigen was then inoculated subcutaneously into rabbits to stimulate antibody production. The maximum antibody titre was determined by applying indirect ELISA, and was found to have been achieved at one week post inoculation in all groups. It was concluded that with an increase in the dose of LPS antigen, the antibody titre increased and the combination of LPS of P. multocida with yeast produced more immunity when compared to LPS alone in rabbits.
Keywords: Haemorrhagic septicaemia, Immunity, LPS, ELISA Pasteurella multocida
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Print ISSN: 1018-7081

Electronic ISSN: 2309-8694

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