A case-control epidemiological study was carried out to estimate the haematological and serum biochemical risk factors associated with parturient haemoglobinuria on 30 haemoglobinuric and 60 apparently healthy buffaloes selected fromdistrict Chakwal during December, 2010 and January, 2011. Non coagulated and coagulated blood samples were collected and tested for various haematological and biochemical parameters. Significantly (P0.05) difference was recorded between haemoglobinuric and healthy buffaloes with respect to mean corpuscular haemoglobin concentration and serum copper level with an odds ratio of 0.50 and 0.87 respectively. Hienz bodies were not detected on stained blood smears of haemoglobinuric as well as healthy buffaloes. It was concluded that low red cell count, haemoglobin concentration, haematocrit, serum inorganic phosphorous and selenium whereas; increased mean corpuscular volume, mean corpuscular haemoglobin and serum molybdenum content are potential risk factors associated with parturient haemoglobinuria.