During 2006-07, the field experiment was conducted at NSCRI, Thatta to observe the effect of different levels of nitrogen; phosphorus and potassium applications on the yield and quality of sugarcane variety HoTh-300. In this study fertilizer levels of 80 and 160 kg ha-1 P2O5 and K2O, were tested in combination with 200 kg ha-1 nitrogen. The experiment was laid out according to Completely Randomized Design (CRD) with three replications and nine treatments. Significant differences for cane yield and yield contributing traits were observed among the treatments. Highest average cane yield of 123 t ha-1was recorded in T 9 which was followed by 121, 120 and 113 t ha-1 average cane yield in T8, T6 and T5, respectively. Moreover, the plots receiving T7, T4, T3 and T2 displayed average cane yield of 100, 98, 95 and 93 t ha-1, respectively. However, the lowest average cane yield of 86 t ha-1 was recorded from T1 (control). As regards the cost benefit ratio, it was observed that fertilizer dose @ 200+80+80 kg N+P2O5+K2O ha-1 was suitable fertilizer treatment for obtaining maximum cane and sugar yield in genotype HoTh-300.