The objective of this study was to determine the effect of GnRH and estradiol benzoate (EB) on follicular wave emergence, estrus, ovulation and pregnancy rate in CIDR treated buffaloes. Buffaloes were randomly selected into three treatment groups which received either GnRH, (100 μg; Dalmarelin; Fatro, Italy; 2 ml, i.m.); (n = 9) or EB (3 mg plus 50 mg progesterone; Duoton Fort; T.P Drug, Lab., Thailand; 2 ml; i.m.); (n = 6) along with insertion of CIDR (day 0) while buffaloes (n = 10) with CIDR alone served as control (CON). All Buffaloes were administered PGF2α, (0.150 mg; Dalmazine, Fatro, Italy; 2 ml; i.m.) on day 6 followed by removal of CIDR on day 7. Follicular development and ovulation were monitored daily from day 0 until ovulation using real time B-mode ultrasound (Honda; Model: HS-1500; 7.0 MHz). Estrus detection was carried twice daily using penile deviated teaser bull. Buffaloes were once inseminated 12 h after the standing oestrus. Pregnancy diagnosis was carried out on day 45 post insemination using ultrasonography. The results showed that the follicular wave emergence did not differ due to treatments (P > 0.05); however, wave emerged was earlier in buffaloes given GnRH and EB (2.8 ± 0.5 day) than control (4.0 ± 0.5 day). Similarly, the proportion of buffaloes showing estrus did not differ (P > 0.05) among the groups. The interval from PGF2α administration to estrus (64 ± 12.3 h) did not vary due to treatments but was numerically longer in buffaloes given GnRH. The interval to ovulation after estrus (38.6 ± 7.9 h) did not change because of the treatments. There was no significant difference in ovualtory follicle diameter (9.9 ± 0.5 mm) and pregnancy rate (6/19, 30 %) across the treatments. In conclusion, this preliminary data indicate that administration of GnRH and EB result in emergence of follicular wave, estrus and ovulation and similar pregnancy rate in CIDR treated buffaloes.