The present study was designed to determine the miticidal effect of methanolic extract from Vitex negundo Lam. at the rate of 10 and 20% concentrations through topical application on scabies affected skin of camel, buffalo, goat, dog and man. The ivermectin was used as reference compound and100% methyl alcohol as control group. The topical application of the treatment groups on scabies affected buffalos gave 43, 73 ,81 and 0%, on camels 46, 75, 84 and 0% on dogs 42, 77, 83 and 0%, on goats 42, 69, 81 and 0% and on man 61, 78, 84 and 0% protection, respectively, after 5th weeks of application. In addition, the effect of Vitex negundo methanolic extract with three different concentrations (10, 20, and 30%) was studied in vitro to determine the potential to kill Sarcoptes scabiei. The results revealed that 10, 20 and 30% concentrations of Vitex negundo methanolic extract caused 70, 80, and 90% mortality of the Sarcoptes scabiei mites, respectively in comparison to ivermectin and methyl alcohol that gave 85 and 5% mortality, respectively. It is concluded that methanolic extract of Vitex negundo Lam was found good as ivermectin a proven miticide both in vitro and in vivo