This study was conducted to assess the post thawing quality of Kundhi buffalo semen diluted in tris based diluents. Before freezing, the ejaculates were checked for volume, colour, pH, sperm concentration, mass activity, progressive linear motility (PLM), normal morphological characteristics and % of membrane intact cells. The semen qualifying these tests was frozen. The frozen semen after thawing was incubated at 37oC and assessed for progressive linear motility (PLM) and % of membrane intact cells. It was observed that all the ejaculates were creamy white in colour. The mean (±SEM) mass activity, volume, pH, progressive linear motility (PLM), sperm concentration, normal morphological characteristics and % membrane intact cells were. +++, 2.25 ± 0.01 ml, 6.10 ± 0.007, 69 ± 0.34%, 1542 ± 9.20 x 106, 79 ± 1.37%, 55.56 ± 1.37% respectively for fresh semen. No significant difference (P>0.05) was observed between the bulls for the parameters recorded except normal morphological percentages and % of membrane intact cells where a significant difference (P<0.05) was observed. The mean (±SEM) post thawing progressive linear motility (PLM) and %of membrane intact cells was found to be 43±3.40% and 31.4 ± 3.07% respectively. A significant difference (P<0.05) was observed between the bulls for post thawing semen parameters. It was concluded that semen of Kundhi bulls tolerate freezing and thawing stress and maintained reasonably good score of parameters to be considered for A.I programme. The objective method Osmotic Resistance Test (ORT) was found to be useful parameter for assessment of in vitro quality of buffalo semen.