his study was designed to determine conception rate in Kundhi buffaloes treated with prostaglandin F2 alpha (PGF2α) after timed insemination and simultaneous treatment with or without gonadotrophin releasing hormone (GnRH). A total 40 anestrus Kundhi buffaloes were randomly selected in the vicinity of Sindh Agricultural University, Tandojam and were divided in to 4 equal groups, each comprising of ten animals (n=10). Animals in group A, were injected intramuscularly with 2 ml Dalmazine, a synthetic analogue of prostaglandin PGF2α (2 ml Dalmazine = 0.150 mg cloprostenol, Fatro, Itly), on day 1 followed by the same treatment on day 11 with fixed time artificial insemination (72 h) after the 2nd dose of prostaglandin injection. In group B, same treatment as in group A but in addition gonadotrophin releasing hormone (GnRH) @2 ml of Dalmarelin (Lecirelin aetate Fatro, Itly), a synthetic analogue of GnRH was injected at the time of fixed time AI. In group C, same protocol as in group A, but with 2 ml of Dalmazine and 2 ml of Dalmarelin were injected simultaneously on day 11. In group D, animals were kept as control. The conception rate in group A, B, C and D was 20.2%, 30.3%, 30.3% and 10.1% respectively. The mean conception rate in treated group was higher (26.6%) than control (10.1%). It was concluded from the present study that a 16.6% increase in conception rate was achieved in anoestrus Kundhi buffalo through hormonal therapy in field condition.