Article Abstract

Volume 20, No. (3), 2010 (September)
EFFECTS OF GLYCININ AND b -CONGLYCININ ON INTEGRITY AND IMMUNE RESPONSES OF MOUSE INTESTINAL EPITHELIAL CELLS
J. Xu, A. Zhou, Z. Wang and D. Ai

J. Xu, A. Zhou, Z. Wang and D. Ai

Chengdu Vocational College of Agricultural Science and Technology, Chengdu 611130, P.R. China
*Key Laboratory for Animal Disease –Resistance Nutrition of China Ministry of Education, Ya’an, Sichuan 625014, China

Corresponding Author: xujunsicau@yahoo.com.cn
DOI: NA
Page Number(s): 170-174
Published Online First: June 01, 2010
Publication Date: June 01, 2010
ABSTRACT

Antigen protein contained in soybeans are the major cause of allergic reaction in young animals. In this study, glycinin and β-conglycinin were purified material and experimentally tested at concentration of 0.1, 5, and 10 mg/ml for their effects on integrity and immune responses of mouse intestinal epithelial cells. At 5 mg/ml or above, glycinin and β-conglycinin undermined the integrity of intestinal epithelial cells, inhibited epithelial cell proliferation, and significantly increase the levels of LDH and GOT released to the culture medium. The administration of glycinin and β-conglycinin also significantly promoted the secretion of inflammatory cytokines (IL-2, IL-6, IL-8), a key feature in allergic reactions.

Keywords: Glycinin, β-conglycinin; intestinal epithelial cells; integrity; cell factor
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Electronic ISSN: 2309-8694

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