Effect of feeding frequency on growth and survival of Labeo rohita (rohu), during fingerlings rearing was studied in 280 L glass aquaria. Feeding frequency one, two and three times daily as three treatments were evaluated against a control without feeding in triplicate glass aquaria in a trial of 60 days. Single breed fingerlings ranges from Mean length 6.8±0.3 cm, Mean weight 4.18±0.02 g were stocked at the density of 25/aquarium. Survival rate were recorded as 100 %, Similarly, the growth in terms of length and the feed conversion ratio did not differ significantly (P > 0.05) among treatments and were in the ranges of 7.92 – 13.6 cm and 1.39 - 1.54 respectively. The study suggests that feeding frequency of three times daily is sufficient for under intensive rearing culture of Labeo rohita fingerlings.